GloQube Glow discharge system

from Quorum Technologies

The GloQube is a compact, easy to use, stand-alone glow discharge system. The primary application of the system is the hydrophilisation (wetting) of carbon-coated TEM support films and grids. Glow discharge treatment with air will make surfaces negatively charged and hydrophilic and allow the spread of aqueous solutions.

Features
Dual or single chamber
Hydrophilic/hydrophobic and negative/positive modes
Fully automatic, short process times, touch screen control
Safe vapour delivery using septum-sealed vials
Automatic valving between chambers to prevent cross-contamination

The GloQube is a compact, easy to use, stand-alone glow discharge system.

The primary application of the GloQube is the hydrophilisation (wetting) of carbon-coated transmission electron microscopy (TEM) support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air/oxygen will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.

Surface stateChargeAtmosphereTypical applications
HydrophilicNegativeAirCarbon coated TEM grids
HydrophilicPositiveAir with magnesium acetate post-treatmentNucleic acid adhesion to carbon films
HydrophobicPositiveAlkylamineProteins, antibodies and nucleic acids
HydrophobicNegativeAirPositively charged protein molecules, (e.g. ferritin,
cytochrome c)

The GloQube® is available in two formats: The GloQube-D has two independent vacuum chambers: a clean chamber for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas, and a vapour chamber designed for hydrophilic/hydrophobic (negative or positive) conversions, typically using reagents such as methanol and alkylamine. The GloQube-S has a single clean chamber.

With operator safety and ease of use firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is both convenient and reliable and ensures minimal user contact with the reagents. The vial is located in its holder and inserted into a shielded needle using a simple twist-and-push home motion.

Each chamber can accommodate two 25 x 75 mm glass microscopes slides. Loading could not be easier using draw-style chamber doors and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. For additional convenience – and to allow easy access for chamber cleaning – the stages can be completely removed.

The GloQube-D has automatic valving between chambers which maintains cleanliness by preventing cross-contamination. At the end of a process run, automatic "soft" venting to atmosphere through filtered inlets ensures that delicate samples, such as TEM grids, are not disturbed.

Both GloQube-D and GloQube-S require a single vacuum pump working in the 0.1 to 1 mbar range. A typical pump time to operational vacuum is 60 seconds. A 750 mm flexible stainless vacuum hose is supplied with the GloQube.

Plasma current 1-40 mA
HV power supply 30 W
Maximum voltage 800 V
Electrode polarity – clean chamber DC glow positive DC glow negative
Electrode polarity – vapour chamber DC glow positive DC glow negative
Sample stage125 x 100 mm (4.9” x 3.94”) for two 25 x 75 mm (1” x 3”) slides
Sample stage operational heightsAdjustable 12.5 mm (0.5”), 22.5 mm (0.9”) or 35 mm (1.38”)
Pump hold time0-24 hours
Process time1-600 seconds
Vacuum control/ working rangeIntegrated pirani gauge, 0.1 to 1 mbar
Pump minimum requirements 6 m3/hr, 3600 l/m, 0.03 mbar ultimate vacuum.
chamber vent inlets Filtered air inlets with slow vent to minimise sample disturbance
On-board reagent storageReagents are contained in reusable sealed glass vials to minimise exposure to hazards
High voltage safety interlocksHardware safety interlocked and software for process control
Chamber size 100 mm W x 100 mm H x 127 mm D (3.94” x 3.94” x 5”)
Instrument size/weight366 mm W x336 mm H x 364 mm D (13.2” x 14.3”) 19.5 kg (42.9 lbs.) (GloQube-D)
Power requirements120 V 60 Hz, 15 A or 230 V 50 Hz, 10 A
Communication portEthernet port for instrument software updates
Surface Modifikation of carbon coated TEM – Grids and films
Cleaning of glass slides
Surface Modifikation (Hydrophil/Hydrophob)

Contacts

Request further information
Product Manager - Electron microscopy & nanotechnology
+49 6151 8806-12
Fax: +49 6151 8806912

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